Immortalization of DM1 primary myoblast populations can also be achieved by combined ectopic expression of TERT, mutated CDK4(R24C) and cyclin D1 (CCND1), which increases the CDK4 kinase activity for efficient Rb hyperphosphorylation (Pantic et al., 2016; Shiomi et al., 2011). The gene discussed is RB1; the disease is myotonic dystrophy type 1.