Next, we analyzed apoptosis in Dvl2-overexpressing RA-FLSs using Annexin V-APC/7-AAD staining (Figure 4C, 4D) and found increased levels of apoptosis in Dvl2-overexpressing RA-FLSs compared to control-transfected RA-FLSs. This evidence concerns the gene ANXA5 and rheumatoid arthritis.