Fig 3C shows that there was a significantly lower number of CD4+CD25+Foxp3+ T regulatory cells induced by DC-based HHP lung cancer vaccine when compared to iDC and DC pulsed with HHP-killed tumor cells alone. Poly(I:C) stimulation alone decreased the number of CD4+CD25+Foxp3+ T regulatory cells, however the number of CD4+CD25+Foxp3+ T regulatory cell was still slightly higher than the number of T regulatory cells induced by the DC-based lung cancer vaccine (Fig 3C). This evidence concerns the gene CD4 and neoplasm.