In our retrospective study, we revealed that PSA and AKP were notable higher in PCa patients than that in BPH patients, and it has come to our attention that larger prostate size, more scleroid texture and more tubercle existed, and more central sulcus disappeared during digital rectal examination in PCa, further, more abnormal echo, lesion and signal existed in ultrasonography, computed tomography and magnetic resonance imaging (MRI), respectively. This evidence concerns the gene KLK3 and benign prostatic hyperplasia.