Our present study has shown that CAFs induced the phosphorylation of JAK2 and STAT3 of gastric cancer cells via the secretion of IL-6, and inhibiting JAK2-STAT3 pathway activation with AG490 significantly impaired cell migration and EMT, as well as peritoneal dissemination and metastasis in vivo induced by CAFs. This evidence concerns the gene STAT3 and gastric cancer.