The best keeper revealed that in DNBS induced colitis the highest stable genes were Tbp (0.305), Rplp0 (0.440), Hprt (0.574), Eef2 (0.905) and Nono (0.920), and while the least stable genes were Actb (0.934), Gapdh (0.967), Ppia (1.242), Oaz1 (1.246), Gusb (1.296), Hmbs (1.368), Trfc (1.439) and β2m (1.567). This evidence concerns the gene GAPDH and colitis.