For example, mutations in EGFR and MLL2 were private to 327_T_PDO, whereas tumour 278_T and its models diverged for mutations in FAM123B, MTOR, and for a PTCH1 frameshift mutation predicted to activate oncogenic sonic hedgehog signalling (Fig. 3b; Supplementary Data 6). Here, PTCH1 is linked to neoplasm.