LEF1 and neoplasm: To investigate metabolic changes within the tumor, 5.0‐ to 6.0‐μm serial sections of formalin‐fixed, paraffin‐embedded tumor were probed with antisera specific for phosphorylated PDH (pPDH) as an indicator of PDK activity, and lymphoid enhancer factor‐1 (LEF‐1), a Wnt signaling transcription factor and Wnt target gene (Hovanes et al, 2001).