To further evaluate if the antibodies induced by P467-CRM-Montanide or with P467-CRM-Alum bind to native Her-2/neu, sera of the immunized mice were used for staining of Her-2/neu overexpressing breast cancer cell line SK-BR-3 or the human melanoma cell line 518.A2 as control cells (Her-2/neu negative), and analyzed by FACS. The gene discussed is ERBB2; the disease is breast carcinoma.