Using this approach on fresh frozen, PFA-fixed sections (from 5 normal brains and 5 PD brains supplied by the Parkinson's UK Brain Bank; Table 1), we successfully investigated CD24 expression across 9 regions of the brain: medulla, pons, midbrain, amygdala, thalamus, subventricular zone (SVZ), hippocampus and frontal cortex. The gene discussed is CD24; the disease is Parkinson disease.