It should be noted that the peak inhibitory effect of p38 inhibitor (SB203580) on cyclin D1 expression was reached at 6 h, whereas ERK inhibitor (U0126) treatment resulted in a delayed inhibitory effect (at 24 h) on cyclin D1 expression in HCC cell lines (Figure 4g). This evidence concerns the gene CCND1 and hepatocellular carcinoma.