RAE1 and breast cancer: To investigate whether RAE1 deficiency would induce the opposite effects in breast cancer cells, we generated stable RAE1-knockdown MCF7 and MDA-MB-231 cells (MCF7:shRAE1 #1, 2, 3, 4 and MDAMB231:shRAE1 #1, 2, 3) and control cell lines (MCF7:sh NS #1, 2 and MDAMB231:sh NS #1, 2) after infection with lentiviral particles containing RAE1 shRNA and selection.