TMSB10 and breast cancer: To further confirm the results from public datasets, we examined the expression of TMSB10 in two normal breast epithelial cell lines and eight breast cancer cell lines by real-time PCR and western blotting, and found that mRNA and protein levels of TMSB10 were upregulated in breast cancer cells compared with NMEC1 and NMEC2 (Fig. 1d and e).