PTS and type 2 diabetes mellitus: A similar trend (i.e., a significant association with T2D only when applying the revised pipeline) was also found for several other pathways such as the phosphotransferase system (PTS; ko02060) and the Biofilm formation-vibrio cholerae (ko05111; see Additional file 9: Figure S7), both of which were found to be associated with high levels of sugar in the blood [12].