In the present study, siRNA approaches were employed for KPNA2 functional analysis in vitro and in vivo because KPNA2 was expressed in all cholangiocarcinoma, gastric cancer, and colon cancer cell lines in our department, and an association between KPNA2 suppression and cell proliferation was noted in cholangiocarcinoma cells. Here, KPNA2 is linked to colonic neoplasm.