Signaling pathway analysis revealed that LDLR and 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), which are involved in cholesterol uptake and synthesis, respectively, were down-regulated in a dose-dependent manner, suggesting the possibility that acRoots inhibited cholesterol uptake and synthesis by altering LDLR and HMGCR expression in HCC. The gene discussed is LDLR; the disease is hepatocellular carcinoma.