CD4 and neoplasm: Intriguingly, increases of splenic immune cells such as CD4+, CD8+ T and NKT cells or decrease of splenic CD4+CD25+FoxP3+ regulatory T cells after MART1 plasmid + AdMGshT were not significant compared to those of tumor-infiltrating immune cells, which provides the possibility that intratumoral injection can induce more rapid and potent immune activation in tumor site than in spleen.