CD163 and breast cancer: As illustrated in Figure 1B, immunofluorescence revealed a barely detectable immunostaining of CD68 in CAFs (Figure 1B, d) and NHDFs (Figure 1B, g) meanwhile no expression can be detected for CD163 and F4/80 markers in CAFs (Figures 1B, e and f) and in NHDFs (Figures 1B, h and i), ruling out that the cells prepared from breast cancer tissue are not macrophages.