TWIST1 and colorectal cancer: To determine the prognostic significance of this potential correlation, we used IHC to analyze APA-TWIST co-expression in colorectal cancer samples, and found (a) significantly higher levels of APA and TWIST protein in tumor tissue compared to paired normal tissue (Figure 6C) and (b) large elevations of TWIST expression in nuclear bases associated with upregulated APA in tumor stroma (Figure 6D, upper).