The analysis indicated that ALS CSF-driven reduction in luminescence in NSC-34-Gli cells (Fig 2E) was associated with significantly reduced nuclear intensity of Sufu (34.13 ± 8.55, n = 520 in controls versus 28.44 ± 5.63, n = 429 in ALS group, p < 0.0001, t-test) (Fig 2F), suggesting its potential entrapment in the cytoplasm in complex with other factors such as Gli1, as reported before [52]. Here, GLI1 is linked to amyotrophic lateral sclerosis.