In agreement with results from Shh-LTII cells, the luminescence output was also significantly reduced in ALS CSF-treated NSC-34-Gli cells, compared to light output from control cells (p = 0.0412) (Fig 2E) indicating that this ALS CSF-driven inhibition is not restricted to a single cell type. The gene discussed is GLI1; the disease is amyotrophic lateral sclerosis.