Specifically, images from ALS-CSF-treated cells (ALS panel) stained for Gli1 and Sufu show more pronounced cytoplasmic and perinuclear cuffing of Gli1 (green) and Sufu (red), resulting in orange color present in the cytoplasmic area in the merged Gli1/DAPI/Sufu channel. The gene discussed is CSF2; the disease is amyotrophic lateral sclerosis.