LS174T and DLD1 colon cancer cell lines were used because they express low endogenous levels of wild type or compound heterozygous mutant (T719I and M847K) EPHA3 protein18, respectively (Supplementary Figure 2A), and high levels of the preferred EPHA3 ligand EphrinA5 (Supplementary Figure 2B). Here, EPHA3 is linked to malignant colon neoplasm.