First, we investigated whether the reintroduction of EPHA3 into LS174T and DLD1 colon cancer cells modulated their growth in vitro by directly counting the number of cells at different times post-seeding, and found no differences after EPHA3 overexpression by doxycycline treatment (Fig. 2A,B). The gene discussed is EPHA3; the disease is colonic neoplasm.