We found the target genes of the up-regulated miRNAs were mainly enriched in six metabolic pathways including cancer signal pathway, insulin signal metabolic pathway, cell binding molecules, melanin generation, long-term regression and P53 signaling pathway (as shown in Fig. 5), and the number of genes enriched in cancer signaling pathway was the largest, suggesting that the up-regulated miRNAs affected pathogenesis process of MM through a variety of ways and participating in various metabolic pathways after regulating the expression of target genes. This evidence concerns the gene TP53 and cancer.