ANXA5 and head and neck squamous cell carcinoma: To investigate the apoptotic effect of CA and CAPE, Detroit 562 cells were treated with both substances for 24 h and 48 h, and apoptotic cells were assessed by staining with Annexin V. To determine whether CA/CAPE treatment results in apoptosis in Detroit 562 HNSCC cells, we used a Muse Annexin V and Dead Cell kit to measure the changes in cell apoptosis after 24 h and 48 h.