CD8A and infection: The MHC I tetramer staining showed the same trends as observed before the challenge infection, with a still severely impaired GagL85–93-specific CD8+ T cell response in the mice immunized with Ad.pIXg70 followed by Ad.TxnGagL, which was comparable to that observed in unvaccinated mice, demonstrating that there was no rapid anamnestic proliferation of vaccine-induced GagL85–93-specific CD8+ T cells (Fig. 2b).