To confirm that KLF6 is a transcriptional activator of the four NF-κB-negative regulators, LN229 glioblastoma cells and two brain tumor-derived stem-like cells (BTSCs) (BTSC23, BTSC233) were transduced with a lentivirus expressing empty vector (EV), KLF6-wt or the non-functional KLF6 variant sv1 as a negative control24 (Figures 3a–c and Supplementary Figures 3A–B). Here, NFKB1 is linked to brain neoplasm.