As hepatic SHARPIN expression was significantly downregulated in the CCl4 and APAP induced acute liver injury models (Figure 1c), as well as in the MCD-diet fed NASH model [12], we first knocked down SHARPIN in the liver by injecting C57BL6/J mice with SHARPIN shRNA expressing adenovirus (Ad-SHARPIN shRNA) via the tail vein. The gene discussed is SHARPIN; the disease is metabolic dysfunction-associated steatohepatitis.