To investigate whether the decrease in HIF-1α expression by CA in hypoxic prostate cancer cells involves in the SPHK-1 pathway, AKT, and GSK-3β and to delineate the downstream signaling mechanism of SPHK-1, we performed western blot analysis and SPHK-1 activity assay. The gene discussed is HIF1A; the disease is Familial prostate cancer.