AGO2 and posterior cortical atrophy: To identify mRNAs differentially enriched or excluded from the RISC complex, Ago-2 was immunoprecipitated from extracts derived from normal prostate fibroblasts (PNF-08) and from the PCa cell line DU145 using the previously described Ago-2 antibody 11A9 and an appropriate isotype control [15, 16].