ACTB and nonpapillary renal cell carcinoma: The relative abundance of RASSF1A mRNA was calculated as the ratio of RASSF1A mRNA to β-actin mRNA and was found to be nearly three times less in ccRCC tissue (0.032 ± 0.009) than in normal tissue (0.0923 ± 0.028) (p < 0.05) (Figure 4C).