Therefore, in this study, we aim to firstly identify tissue Hp level difference (between adjacent non-tumorous tissues and tumor tissues) as a prognostic marker for HCC outcomes using two cohorts, including prospective patients sample collections (n = 130) from Changhua Christian Hospital (CCH) and Hp mRNA analysis (n = 316) from The Cancer Genome Atlas (TCGA) database. This evidence concerns the gene HP and hepatocellular carcinoma.