In fact, we highlighted a significant mRNA down-modulation of TWIST1, Fibronectin1 (FN1) and Vimentin1 (VIM1) in shHes1-CSC, indicating a role of Notch1 in defining the molecular profile of GBM mesenchymal subtype (Figure 4B). The gene discussed is NOTCH1; the disease is glioblastoma.