To investigate the effect of si-ABHD11-AS1 and pcDNA3.1-ABHD11-AS1 on the apoptosis of bladder cancer cells, the caspase-3 enzyme-linked immunesorbent assay (ELISA), Hoechst 33258 staining assay and flow cytometry were used to measure the apoptosis rate. The gene discussed is CASP3; the disease is urinary bladder cancer.