The pathway analysis (Fig. 6b) suggest that IL-1β globally regulated 76 genes play significant roles in various molecular/cellular functions including cellular movement, cell-to-cell signaling and interaction, protein synthesis, cellular function and maintenance, and cell death and survival, which may also contribute to the development of inflammatory response, connective tissue disorders, inflammatory disease, skeletal and muscular disorders, and immunological disease. This evidence concerns the gene IL1B and connective tissue disorder.