Consistently with our results in the TCGA database and meta-analysis, the results from the tissue microarray demonstrated that AEG-1 was overexpressed in NSCLC compared with normal lung, and there was a significant correlation between AEG-1 expression and tumor size, clinical stage and lymph node metastasis, which are all in agreement with the previous findings of Ren et al. [16]. This evidence concerns the gene MTDH and neoplasm.