BIN1 and esophageal squamous cell carcinoma: ESCC patients with poor differentiation grade, high TNM stage (stage III+IV), deep tumor invasion (T3), and positive lymph node metastasis had significantly higher rate of methylated Bin1 than did those with well or moderate differentiation grade, low TNM stage (stage I and II), superficial tumor invasion (T1 and T2), and negative lymph node metastasis (Table 1).