cDNA libraries were derived from the SNU-484 human gastric cancer cell line, the SNU-81 human colon cancer cell line, and the HEK293 normal human kidney cell line, and calgranulin B-interacting partners were screened using the bait plasmid, pGBKT7-calgranulin B. Interactions of bait and prey proteins were examined by assessing growth on two selective media with different levels of restrictiveness: TDO (-His/X-a-Gal/AbA/) and QDO (-His/-Ade/X-a-Gal/AbA) (Fig 2). This evidence concerns the gene S100A9 and gastric cancer.