LZTR1 and infection: Importantly, viruses encoding mutations in the NS4A-kink region (NCP7 NS2-Ubi-NS3 (2/E440V + 4A/L45A); NCP7 NS2-Ubi-NS3 (2/E440V + 4A/Y47A)) instead of 3/V132A were also able to reach high titers at 48 h pe (4A/L45A: 4.0 x 105; 4A/Y47A: 1.1 x 106) or after defined infection at MOI 0.1 (4A/L45A: 6.0 x 105; 4A/Y47A: 2.0 x 106) (Fig 5C).