We confirmed that AR was highly expressed in the androgen-responsive prostate cancer cells (LNcap cells) but was hardly detectable in the androgen-irresponsive prostate cancer cells (DU145 and PC3 cells) tested here (Figure 1a), implying that the induction of cell cycle arrest by USP14 inhibition is AR dependent. This evidence concerns the gene USP14 and Familial prostate cancer.