TFF3 and cancer: However, pathologic TFF3 expression exerts similar effects in different cancers and promotes metastatic seeding.25,34 Human intestinal adenocarcinoma cells HT-29/B6 used in this study grow as polarised monolayers and represent a well-established in vitro model for intestinal cell biology.35 Several studies suggested a close connection of TFF3 to PI3K/AKT and Wnt pathways, both commonly reported to be dysregulated in cancer.