Our initial study of CNAs in Ewing sarcoma identified a focal region of amplification in a subset of Ewing sarcoma tumors that contained five genes: RNF114, SNAI1, UBE2V1, TMEM189, and CEBPB. We used the same dataset (GSE1825) to evaluate expression of all five genes within the focal region of high copy gain in the Ewing sarcoma samples compared to neuroblastoma samples. The gene discussed is RNF114; the disease is Ewing sarcoma.