To further examine the necessity of FoxM1 expression for the Gli1-mediated proliferation of CRC cells, we performed complementary experiments by separately and simultaneously treating LoVo cells, which moderately expressed both endogenous FoxM1 and Gli1 proteins, with the Hh-Gli1 activator purmorphamine and the FoxM1 inhibitor thiostrepton [39]. This evidence concerns the gene GLI1 and colorectal carcinoma.