LGR5 and intestinal neoplasm: We analyzed the expression of tumor stem cell markers, and found a massive increase in mRNA and protein levels of the Dclk1, Lgr5, Bmi1, and Musashi1 (Fig. 2d) in the IECs of ApcMin/+ mice compared with WT control mice, corroborating the presence of highly tumorigenic cells in the Apc mutant intestinal tumors.