ESR2 and neoplasm: When we considered methylation of at least one of four genes (MCAM, ERα, ERβ and SSBP2) in our tested serum samples (tumor cases and controls) similar sensitivity as of sPSA was obtained (77% for sPSA versus 78% for gene combination), however, the specificity was increased by combination gene panel (30% for sPSA versus 53% for 4 genes combination) (Figure 3).