As compared to control THP1 cells expressing YFP (THP1Ctrl#1), THP1YFP-UBE2L3 cells showed diminished LPS-induced pro-IL-1β (Figure S5A) and therefore released ∼2- to 6-fold less IL-1β after nigericin treatment or STm infection (Figure 4A). This evidence concerns the gene IL1B and infection.