Apart from potential driver genes, we investigated a number of recurrently mutated genes in our cohort: three genes known to be frequently mutated in cancer (EPHA2, BAP1, MUC16) as well as three genes neither frequently reported in biliary tract cancer nor present in cancer gene census (DCAF4L2, C11orf65, ABCC9) were found. Here, C11orf65 is linked to biliary tract cancer.