As expected, HFD-induced obesity resulted in a significant increase in expression of inflammatory markers including the pro-inflammatory cytokine TNFα27, the chemokine monocyte chemoattractant protein (MCP)−128, the monocyte/macrophage markers CD11b, CD11c, CD68, and F4/8029 (Fig. 6a–f). Here, ITGAM is linked to obesity due to melanocortin 4 receptor deficiency.