TSC2 and fragile X syndrome: After sorting all subjects by their Ca2+ signaling normalized to the reference cell line (as was done for Fig. 2), we generated an ROC curve by plotting sensitivity (true positive rate) against 1-specificity (false positive rate) at each test value for individuals with syndromic ASD (FXS, TSC1 and TSC2, Rett and PWS) as shown in Fig. 3a.