To determine whether Wee1 downregulation influenced the effect of the chemotherapeutic drug doxorubicin in osteosarcoma cells, KHOSMR and U‐2OSMR cells were transfected with Wee1 siRNA and then incubated with doxorubicin, a commonly used first‐line chemotherapy drug in the treatment of osteosarcoma. The gene discussed is WEE1; the disease is osteosarcoma.