As MET amplification has been implicated as a mechanism by which secondary resistance EGFR TKI can emerge in NSCLC, we used a MET/cep7 fluorescence in situ hybridization (FISH) probe and calculated the ratio of MET to chromosome 7 centromere signals and the mean copy number of MET. The gene discussed is EGFR; the disease is non-small cell lung carcinoma.