Mice challenged with S. pneumoniae displayed disruption of BBB integrity with significantly increased albumin (Fig 7A) and IgG (Fig 7B) in brain homogenates and showed moderate severity of disease with substantially decreased spontaneous motor activity (Fig 7C) and increased body weight loss (Fig 7D) at 30 hrs after infection (p<0.01 versus mice treated with PBS), whereas administration of B7-H3 further aggravated BBB disruption (Fig 7A and 7B) and disease severity (Fig 7C and 7D) in S. pneumoniae-infected mice (p<0.05 versus mice challenged with S. pneumoniae alone). This evidence concerns the gene CD276 and infection.